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Independent research

Six people. A jar of peanut butter a day. Zero fat gained.

Independent, double-blind, 14-day overfeeding study. Same total calories. Different outcome.

4.6 across 2,700+ verified reviews ·Made in USA ·70,000+ customers ·Since 2008
14-day DXA measurement
Regular PB+1.00 kg fat mass
WiO PB0 kg

Same calories. Different outcome. (p < 0.05)

Study design

Independent. Double-blind. Crossover.

Each subject completed both arms: regular peanut butter for 14 days, then WiO peanut butter for 14 days (or the reverse). A washout period reset baseline in between. Subjects, staff, and the laboratory were all blinded to assignment.

01

Recruit

6 healthy adults. Baseline metabolic-cart testing established each subject's daily caloric needs before the study began.

02

Arm A · 14 days

One 12 oz jar (340 g) of regular peanut butter daily, on top of normal diet. All overfeed blinded to peanut butter assignment.

03

Washout

Normal diet, no peanut butter. Baseline reset before the second arm to eliminate carry‑over effects.

04

Arm B · 14 days

Same 12 oz jar (340 g) daily, same normal diet. Identical total caloric intake. Different fiber composition.

05

Measure

DXA scan for body composition. Resting metabolic rate via cart so any change shows. Respiratory exchange ratio measured. Dietary tracking three days per week.

06

Compare

Within-subject fat mass delta calculated. Each participant served as their own control, which strengthens statistical power despite the small sample size.

Conducted atApplied Science and Performance Institute, Tampa, FL
DesignDouble-blind crossover
Subjects6 healthy adults
Duration14-day per arm

An n of 6 is small for an absolute claim. The crossover design partially compensates, because each subject served as their own control. The primary finding (the mass difference) was statistically significant (p < 0.05). The broader cyclodextrin mechanism has been validated in larger studies across multiple populations. See the full reference list.

Study result

Fat mass changed. Metabolism did not.

Calorie intake was matched between groups with no significant differences in total intake, fat percentage, carb percentage, or protein percentage consumed. The only meaningful difference was in fat mass at the end of each 14‑day arm.

Regular Peanut Butter
+1.00 kg
340 g per day, overfed for 14 days
  • +1.00% body fat increase
  • DXA-confirmed fat-mass gain
  • No change in resting metabolic rate
WiO Peanut Butter
0 kg
Same 340 g per day, same 14 days
  • No measurable fat-mass gain
  • Resting metabolic rate unchanged
  • No significant change in lean mass
Same calories. Different outcome.

Regular peanut butter group gained significant fat mass (mean difference +1.00 kg, 95% CI 0.11 to 1.88 kg, p < 0.05). WiO group showed no significant fat-mass change. No significant differences in resting metabolic rate or respiratory exchange ratio between conditions. This rules out a metabolic‑rate explanation: the mechanism is fat binding, not calorie burning.

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The study product

The peanut butter the study tested.

Same recipe, same fiber. Available now. Try it the way the study subjects did, as part of your normal routine.

STUDY PRODUCTWiO Creamy Peanut Butter jar
4.6(232) Verified buyers

WiO Creamy Peanut Butter

31 cal·1g net carbs·7g protein
$12.59SAVE 10%
$13.99 one-time
STUDY PRODUCTWiO Chocolate Peanut Butter jar
4.2(92) Verified buyers

WiO Chocolate Peanut Butter

34 cal·1g net carbs·6g protein
$12.59SAVE 10%
$13.99 one-time
The whole lineup

Same fiber, every product.

The study tested peanut butter because the experimental design needed a high-fat overfeeding condition. Peanut butter was the right vehicle. But the same modified cyclodextrin is in every WiO product: bread, pizza crust, cookies, croissants, tortilla chips. The fiber does the same work in each.

Read how the fiber works
Study questions

What people ask about the study.

Was the sample size big enough?
Six subjects is small for an absolute claim, and we say so plainly. The crossover design helps: each person served as their own control across both arms, which raises statistical power for a within-subject comparison. The primary fat-mass difference reached significance (p < 0.05). The underlying cyclodextrin mechanism has also been validated in larger trials, listed in the reference section below.
Was the study peer-reviewed?
The full journal citation is pending publication. The study was conducted at the Applied Science and Performance Institute in Tampa, FL, with the methods summarized in the protocol above. The cyclodextrin mechanism it relies on is supported by the peer-reviewed research listed in the citation block.
How soon will I see results?
The study measured a 14-day window under deliberate overfeeding conditions, which is not how most people eat day to day. WiO is a food you swap in, not a program with a timeline. We avoid promising a specific outcome on a specific date.
Is the WiO peanut butter safe?
Modified cyclodextrin is a soluble dietary fiber that has been used in food and pharmaceutical applications for decades. WiO products are made in the USA. As with adding any fiber to your diet, some people notice digestive adjustment in the first few days.
Where can I read the full study?
The protocol summary and the primary citation are in the citation block below. The full journal write-up is pending publication; methods and the related cyclodextrin research are available now through their respective journals or PubMed.
Which WiO product should I start with?
If you want to match the study, start with the peanut butter. If you want the everyday version of the same fiber, the Multigrain Bread is the most popular first order. Both carry the same modified cyclodextrin.
Citation

How to cite this study.

Primary study

Applied Science and Performance Institute. Effects of overfeeding regular versus modified peanut butter on body composition in healthy adults: a double-blind crossover trial. Tampa, FL.

Full journal citation pending publication. Study conducted at ASPI, Tampa, FL. Methods available in the protocol summary above.

Related cyclodextrin research

Six peer-reviewed studies supporting the cyclodextrin mechanism. All publicly available through their respective journals or PubMed.

  1. Grunberger G, Jen KL, Artiss JD. (2007). Benefits of early intervention in obese diabetic patients with new dietary fibre, cyclodextrin. Diabetes Metab Res Rev. 23(1):56–62.
  2. Buckley JD, Howe PR. (2013). Acute reductions in dietary fat absorption with alpha-cyclodextrin in healthy adults. Br J Nutr. 110(7):1278–1284.
  3. Comerford KB, Artiss JD, Jen KL, Karakas SE. (2011). Beneficial effects of alpha-cyclodextrin on blood lipids and weight in healthy humans. Obesity (Silver Spring). 19(6):1200–1204.
  4. Gallaher DD, Gallaher CM, Plank DW. (2007). Alpha-cyclodextrin selectively increases faecal excretion of saturated fats. FASEB J. 21:702.
  5. Jarosz PA, Fletcher E, Elserafy E, Artiss JD, Jen KL. (2013). Effect of alpha-cyclodextrin on postprandial lipid and glycemic responses to a fat-containing meal. Metabolism. 62(10):1443–1447.
  6. Artiss JD, Brogan K, Brucal M, Moghaddam M, Jen KL. (2006). Effects of a new soluble dietary fiber on weight gain and selected blood parameters in rats. Metabolism. 55(2):195–202.
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